8 Oct 2020 - 8 Oct 2020
Omics, pathways and modes of action: "Use of differential gene expression analysis, pathway enrichment, visualization tools and bioinformatics resources to infer modes-of-action (MOAs)"
When: Thursday, October 8, 2020 @ 12pm EDT
Presenter: Dr. Mel Andersen
Differential gene expression analysis (DEGA) has proved useful for assessing modes of action (MOAs) with various compounds both following in life exposures to intact animals and with in vitro exposures of cells in culture. Various tools have been developed over the past decade or so to assist in the analysis of these patterns of differential gene expression, including heatmaps, pathway enrichment analysis, benchmark dose estimations and network representations of affected pathways using different ontologies. MOAs inferred from using these gene expression results have sometimes confirmed expectations from conventional toxicity testing and sometimes proved divergent, giving a more comprehensive look at the affected biology with compounds such as styrene, dichloromethane and vanadium pentoxide. In this webinar we discuss modes of action inferred from gene expression studies in relation to dose response modeling, dose-dependent transitions, pathway perturbations and comparing results from one treatment condition with another. The application of these DEGA tools to both in vivo and in vitro patterns of gene expression will likely uncover subtle differences in response among even similar compounds and also provide new tools for assessing the biological consequences of exposures to bioactive compounds. Toxicologists and risk assessors need to be prepared for surprises as these new biological tools are applied to assess MOA even for well-studied compounds.
Scitovation ATTi (Advancement of Transcriptomics for Toxicology initiative)